Inorganic Chemistry, Protein Chemistry, Fluorescence Spectroscopy

1. 06 Year

Post Graduate Level

:

Inorganic Chemistry, Analytical Chemistry

1. Conformational Dynamics Study of proteins/peptides
2. Proteins are the workhorses of cellular machinery. A unique 3D structure of a protein is essential for its proper functioning. However, some proteins lack a stable 3D structure and present as structural assemble known as intrinsically disordered proteins (IDPs). They undergo many functions in organisms through structural and conformational variation. A small or even a single mutation in protein sequence prevents them from proper functioning and may lead to pathophysiological conditions such as sickle cell, Alzheimer’s, Parkinson’s, diabetes, and cancers. Other than mutation, environmental perturbation due to aging also disturbs the conformation of proteins, which may paralyze the proper functioning of proteins. Further, a detailed understanding of the structural or conformational dynamics of proteins in different physiological conditions will give information about their functionality. We study conformers of proteins at various conditions through spectroscopic methods such as steady-state fluorescence, time-resolved fluorescence, and Circular dichroism.

1. University Gold Medal and Late Padma Lalita Memorial Gold Medal, 2015 for securing first position in first class at M. Sc. Chemistry Examination 2015.
2. Lectureship (NET), June 2015 and Graduate Aptitude Test in Engineering (GATE), India, 2015

1. Orissa Chemical Society

International Publications

2022
1. Lipika Mirdha, T Sengupta and H. Chakraborty, Lipid Composition dependent Binding of Apolipoprotein E Signal Peptide: Importance of Membrane Cholesterol in Protein Sorting, Biophysical Chemistry 291 (2022) 106907.
2. Lipika Mirdha and H. Chakraborty, Membrane cholesterol modulates the dynamics and depth of penetration of κ-casein, Journal of Molecular Liquids 363 (2022) 119849.
2021
3. Lipika Mirdha and Hirak Chakraborty, Fluorescence-based techniques for the detection of the oligomeric status of proteins: Implication in amyloidogenic diseases, European Biophysics Journal, 50 (2021) 671-685.
2020
4. Lipika Mirdha and Hirak Chakraborty, Fluorescence Quenching by Ionic Liquid as a Potent Tool to Study Protein Unfolding Intermediates, Journal of Molecular Liquids 312 (2020) 113408.
2019
5. Lipika Mirdha and Hirak Chakraborty, Characterization of structural conformers of κ-casein utilizing fluorescence spectroscopy, International Journal of Biological Macromolecules 131 (2019) 89–96.

International Conferences

1. Lipika Mirdha, Hirak Chakraborty, “Lipid Composition Dependent Binding of Apolipoprotein E Signal Peptide: Importance of Membrane Cholesterol in Protein Trafficking”, 5th Biomembranes symposium 2022, IISc. Bangalore during 15-17th September, 2022 (Poster Presentation)
2. Lipika Mirdha, Hirak Chakraborty, International Symposium on “The Physical Chemistry of Molecular Interactions”, Gangadhar Meher University, India during 12-13th December, 2020.
3. Lipika Mirdha, Hirak Chakraborty, An international webinar series on “Biomolecular Dynamics and Kinetics”, NIT Rourkela, India, 23rd-27th August, 2020.
4. Lipika Mirdha, Hirak Chakraborty, “Characterization of Structural Conformers of κ-casein Utilizing Fluorescence Spectroscopy” 43rd Indian Biophysical Society Meeting (Molecule to system), IISER Kolkata, India during 15 -17th March, 2019. (Poster Presentation)

National Conferences

1. Lipika Mirdha, Hirak Chakraborty, “Understanding the Role of Lipid Composition in Protein Trafficking: A Case Study with Apolipoprotein E Signal Peptide”, 36th Annual Conference of Orissa Chemical Society and National conference on “ Advances in Materials Chemistry and Applications (AMCA 2022) Utkal University, Odisha, India during 18-19th December, 2022 (Oral Presentation)
2. Lipika Mirdha, Hirak Chakraborty, “Lipid Composition Dependent Binding of Apolipoprotein E Signal Peptide: Importance of Membrane Cholesterol inAnnual Conference of Orissa Chemical Society and National Seminar on “ Frontiers in Chemical Sciences (FCS-2021) ,Odisha, India during 18-19th December, 2021 (Poster Presentation)
3. Lipika Mirdha, Hirak Chakraborty, National Conference on Recent Trend in Chemical Sciences (RETICS-2021), School of Chemistry, Sambalpur University during 10-11th December, 2021
4. Lipika Mirdha, Hirak Chakraborty, “Fluorescence Quenching by Ionic Liquid as a Potent Tool to Study Protein Unfolding Intermediates”, National Conference On Recent Trends In Chemical Sciences – 2020, SSN College of Engineering, India, during 26-27th November, 2020. (Oral Presentation)
5. Lipika Mirdha, Hirak Chakraborty, “Probing the Conformations of Intrinsically Disordered Protein Utilizing Fluorescence Spectroscopy” 33rd Annual Conference of Orissa Chemical Society and National Conference on Recent Advancement in Material Sciences (RAIMS–2019), VSSUT Burla, India during 24-25th December, 2019. (Poster Presentation)
6. Lipika Mirdha, Hirak Chakraborty, “Interaction of Ionic Liquids with Intrinsically Disordered Protein: A Case Study with κ-casein”, 22nd Biennial Indian Photobiology Society (IPS) Conference on “Biomolecular Dynamics – Experimental and Theoretical Perspectives (BDETP), NIT Rourkela, India during 18-20th December 2017. (Oral Presentation)
7. “National Conference on Surfactants, Emulsions and Biocolloids (NATCOSEB)”, School of Chemistry, Sambalpur University, Odisha, India during 10-12th November, 2017.

Workshops

1. Lipika Mirdha, Hirak Chakraborty, “Exploring the Mechanism of Guanidinium Chloride-Induced Denaturation of Bovine Serum Albumin: A Fluorescence Spectroscopic Approach”, National Workshop on industry- Academia Interface, Sambalpur University, India during 6-7th March, 2020. (Oral Presentation)
2. Workshop on “Basic Aspects and Applications of NMR Spectroscopy”, IISER Berhampur, Odisha during 24th- 26th September 2018.

1. Lipika Mirdha and H. Chakraborty, Biological membranes: Nature’s own nanomaterial in Bioderived Materials: Nature’s Handyman, Bentham publication (Book Chapter). (In Press)

Present Address :  School of Chemistry Sambalpur University, Jyoti Vihar (Burla), pin -768019

Permanent Address :